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BIOLOGY Services

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What is Biology ?

There is significant need of animal models in biotechnology to mimic human diseases. These models help in understanding mechanism of disease for better therapeutic approaches during the process of drug development.

PRADO has developed and standardized the various animal models of human diseases which plays a crucial role in scientific research and development of health care products. Some of these models are as follows:

In-vitro Biology Assays

Cell Proliferation Assay

  1. DNA Synthesis Cell Proliferation Assays
  2. Metabolic Cell Proliferation Assays
  3. Detecting Proliferation Markers
  4. Measuring [ATP]
  5. Tracking New RNA Synthesis
  6. Fluorescence-Based Cell Proliferation Assays
  7. Measures of Membrane Integrity

Cell Migration and Invasion Assay

  1. Cell Culture Wound-Healing Assays
  2. Transwell Cell Migration and Invasion Assay (Boyden chamber assay)
  3. Cell Exclusion Zone Assay
  4. Fence Assay (Ring Assay)
  5. Microcarrier Bead Assay
  6. Spheroid Migration
  7. Capillary Chamber Migration Assays (microfluidic chamber assays).

Cell Apoptosis Assays

  1. Annexin V Binding Assays by Flow Cytometry Analysis
  2. Caspase Activity Assays
  3. Mitochondrial Membrane Potential Assays
  4. DNA Fragmentation and Morphology
  5. Nuclear Condensation, Non-Caspase Proteases (Cathepsins and Calpain) Activation, Caspase Substrate (PARP) Cleavage, and Cleavage of Anti-Apoptotic Bcl-2 Family Proteins.

Cell Angiogenesis Assays

  1. HUVECs Tube Formation Assay
  2. Rat Aortic Ring Assay
  3. Corneal Angiogenesis Assay
  4. Chick Chorioallantoic Membrane (CAM) Angiogenesis Assay
  5. Matrigel Plug Angiogenesis Assay.

Cell Surface Markers Validation Service, including but not limited to:

  1. Growth Factor, Cytokine, and Chemokine Receptors
  2. Cell Interaction Molecules
  3. Antigen Presentation and Processing
  4. Stem Cell Markers
  5. T cell, B cell NK cell Activation
  6. Analyse cell lines, primary cells, blood samples
  7. Discover new biomarkers
  8. Custom Panels are also available.

Cell-based Screening and Profiling

  1. High-Throughput Screening
  2. High-Content Screening
  3. Specificity Profiling
  4. Selectivity Profiling
  5. Dose-Response Analysis.

 

In vivo Biology Assays

Inflammation and Pain Models

  1. Arthritis Induction using Collagen.
  2. Colitis Induction using DSS or Acetic Acid.
  3. Psoriasis-like Skin Disease.
  4. Excision wound healing model.
  5. Osteoarthritis Induction.
  6. Carrageenan Induced Paw Oedema.
  7. Mechanical, thermal, and cold Hyperalgesia using Complete Freund’s Adjuvant (CFA).
  8. Neuropathic Pain using Paclitaxel.
  9. L-5 & L-6 Spinal Nnerve Ligation (SNL).
  10. Partial Sciatic Nerve Ligation (pSNL).
  11. Glaucoma Model.

Antipsychotic Activity Models

  1. Novel object recognition test.
  2. Psychosis and social interaction deficit.
  3. Disruption of Conditioned Avoidance Response (CAR).
  4. Restoration of pre-pulse inhibition (PPI).
  5. Neurobehavioral assays.

Metabolic Disorders Models

  1. STZ induced Diabetes, Diabetic neuropathy.
  2. Hypothyroidism in rodents.
  3. Acute and Chronic kidney diseases.

Osteoporosis Models

  1. Ovariectomy induced osteoporosis.
  2. Thyroxine or Ethanol induced osteoporosis.
  3. Hypercalcemia using Retinoic acid.

Safety Pharmacology Models

  1. Rota rod test and Grip strength test.
  2. Tail flick test, Hot plate test.
  3. Non-invasive (tail cuff) blood pressure recording.